Antibody Expression and Prodn [Cell Engineering Vol 7]

Abstract Despite having a reputation to be costly, mammalian cell culture processes are used to produce the majority of currently marketed recombinant biopharmaceuticals, many of which are antibodies. Historically, mammalian cells were ainly chosen for whole IgG manufacturing because of product quality requirements like e.g. glycosylation, folding and assembly of the individual chains, which make microbial expression difficult. However, during the past 2 decades, significant progress has been made in both: Speed of mammalian cell line generation and

yield of the manufacturing processes, which makes mammalian systems also from a commercial point of view more and more competitive to microbial expression.The following chapter provides an overview of state of the art mammalian cell line technologies for antibody manufacturing starting from vector and selection systems over host cell lines to screening methods.